Cat. No. P5012-5 ( 5 reactions) Price Inquire
Cat. No. P5012-10 (10 reactions) Price Inquire
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- User Manual  - Technical Bulletin  - MSDS for PhosPro
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Kit contents 10 reactions
LYSIS BUFFER 10ml x 1
DILUTION BUFFER 30ml x 1
NATIVE HOMOGENATION BUFFER 30ml x 1
SOLUTION A 3ml x 1
SOLUTION B 5ml x 1
DISSOLVING SOLUTION 8ml x 1
DELIPIDATION SOLUTION 10ml x 1
Procedure Summary
1. Protein extraction
2. Phosphoprotein specific complex forming
3. Precipitation of phosphoprotein complex
4. Dedelipidation and recovering of phosphoprotein
Phosphoprotein Enrichment from Yeast cell Extract
Phosphoprotein Enrichment from Lung Cancer
Large format phosphoprotein enrichment from SKOV ovarian cancer
The results show that about 1200 spots from the cell can be detected in a single gel with CBB staining (Figure A) and about 700 spots with ProQ Diamond, Phosphoprotein specific staining reagent(Figure B).
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