· Phosphoprotein enrichment from cell/tissue extract
· 2DE, Phosphoprotein staining(ProQ Diamond(Invitrogen)
· Phosphoprotein/phosphorylation site identification by MS analysis
Service item1 Phosphoprotein staining service
Cat. No. P8010 Price
Service item2 Phosphoprotein identification by PMF
Cat. No. P8020 Price
Service item3 Phosphopeptide identification by PSD
Cat. No. P8030 Price
Service item4 Phosphorylation site determination by CAF sequencing
Cat. No. P8040 Price
Service item5 Phosphoprotein enrichment plus Service item1
Cat. No. P8050 Price
Service item1 Phosphoprotein staining service
Cat. No. P8010 Price
Step1
Proteome : total cell lysate
Protein separation : 2-DE mini gel format
1st Dimension IEF : 4-10 NL IPG( 8.5cm)
2nd Dimension slab gel : 8.5 X 8cm slab gel
Staining method :
staining 1 : ProQ Diamond staining (Molecular ProbesTM )
staining 2 : Colloidal CBB staining
Detection method : ProQ fluorescent emission (Ex/Em: 555/580nm)
used filter : Ex/Em: Cy3/Cy3
Step2 : Multiple staining & spot matching
Step3 : Differential display & Comparative analysis
Service item2 Phosphoprotein identification by PMF
Cat. No. P8020 Price
This service provides protein identification and/or candidates of possible phosphorylated peptides
Step1 : Protein identification by peptide mass fingerprinting
Step2 : Prediction of possible phosphorylated peptide by peptide mass matching for phosphorylation modification
Service item3 Phosphopeptide identification by PSD
Cat. No. P8030 Price
Alternative Step1: Prediction of Phosphopeptides by PMF
Alternative Step2(example) : Phosphopeptide enrichment from ProQ-stained spots by Phos-pepTM
Step3 : Phosphopeptide identification by MALDI-TOF PSD
Service item4 Phosphorylation site determination by CAF sequencing
Cat. No. P8040 Price
Step1 : SPITC labeling of Trypsin digest from ProQ-stained spot
Step2 : phosphopeptide Enrichment by Phos-pepTM
Step2 : CAF-sequencing and phosphorylation site determination by MALDI-TOF PSD
Service item5 Phosphoprotein enrichment plus Service item1
Cat. No. P8050 Price
This service provide more chance to meet low abundant phosphorylated proteins
by phosphoprotein-specific enrichment from total protein lysate.
Step1 : Phosphoprotein enrichment from total cell or tissue lysate by Phos-ProTM kit( Genomine’s Proprietary Technology)
Step2 : Phosphoprotein staining service (service item1)
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