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PepFrag™ MALDI Sequencing Kit
   

          MALDI PSD(ESI MS/MS) peptide sequencing
          Protein identification, Phosphorylation site determination

  
         



 

Cat. No. P5011                              Price  Inquire



* Order : mail to  alloyo02@genomine.com


- User Manual            - Technical bulletin   


* User in other countries → Contact Distributors



  

     

       Kit contents            50 Reactions

       Guanidination Reagent                                      0.4ml

       (O-methylisoureahydrogen sulfate salt solution)

       Guanidination Buffer                                            1ml

       (Ammonium hydroxide solution)

       Sulfonation Reagent                                      2 × 2mg

       (4-Sulfophenyl isothiocyanate)

       Sulfonation Buffer                                                1ml


       Procedure Summary

       1. In-gel guanidination (optional)

       2. Trypsin digestion
       3. Sulfonation
       4. Cleaning of modified peptides
 

  



   
    
         

       Sequencing and identification of phosphorylation site of
       Arg- terminated    phosphopeptide 

     


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